CAS No. 108-53-2
Batch:
Purity:99.63
Chemical Structure
Check the
Isocytosine
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| CAS Number | 108-53-2 | |
|---|---|---|
| Molecular Formula |
C4H5N3O |
|
| Molecular Weight (MW) | 111.10 | |
| SMILES | C1=CN=C(NC1=O)N |
Biological Activity
Isocytosine is a cytosine isomer and nucleobase analog used extensively in physical-chemical studies of non-canonical base pairing, metal complex binding, and hydrogen-bonding interactions. It participates in specific hydrogen-bonded base pairs, such as with isoguanine, enabling the expansion of the genetic alphabet and facilitating structural analysis of nucleobase tautomerism and proton transfer dynamics. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
11 mg/mL
(99.0 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.