CAS No. 328998-53-4

Batch: Purity:99.65
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inS3-54-A18 Chemical Structure
Chemical Structure
Check the inS3-54-A18 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (3Z)-3-[(4-chlorophenyl)methylidene]-1-(4-hydroxyphenyl)-5-phenylpyrrol-2-one
CAS Number 328998-53-4
Molecular Formula

C23H16ClNO2
Molecular Weight (MW) 373.83
SMILES C1=CC=C(C=C1)C2=CC(=CC3=CC=C(C=C3)Cl)C(=O)N2C4=CC=C(C=C4)O
InChIKey IMHWQIPPIXOVRK-JXAWBTAJSA-N

Biological Activity

inS3-54-A18 is a small-molecule inhibitor targeting signal transducer and activator of transcription 3 (STAT3) with anti-cancer activity. By inhibiting STAT3 signaling, it suppresses STAT3-dependent transcriptional activation and downregulates downstream target genes involved in cell survival and proliferation. Consequently, inS3-54-A18 attenuates cancer cell viability, induces apoptotic cell death, and impairs oncogenic phenotypes driven by hyperactive STAT3 signaling. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 75 mg/mL (200.62 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 10 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Yu H, et al. Nat Rev Cancer. 2009;9(11):798-809.

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Note: Technical data last updated: Sep 1, 2026.