CAS No. 359010-69-8

Batch: Purity:
Hydroxyphenyl Fluorescein Chemical Structure
Chemical Structure
Check the Hydroxyphenyl Fluorescein product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 359010-69-8
Molecular Formula

C26H16O6

Molecular Weight (MW) 424.40

Ordering Information

Biological Activity

Hydroxyphenyl Fluorescein is a selective fluorescent probe dye designed for detecting highly reactive oxygen species, specifically hydroxyl radicals and peroxynitrite. Upon reaction with these reactive species, the non-fluorescent compound undergoes oxidative cleavage to yield strong green fluorescence with excitation and emission maxima atand, respectively. This enables the specific cellular detection of oxidative stress phenotypes and radical-mediated signaling pathways. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.