CAS No. 625-08-1

Batch: Purity:
3-Hydroxyisovaleric acid Chemical Structure
Chemical Structure
Check the 3-Hydroxyisovaleric acid product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 625-08-1
Molecular Formula

C5H10O3

Molecular Weight (MW) 118.13
SMILES CC(C)(CC(=O)O)O

Ordering Information

Biological Activity

3-Hydroxyisovaleric acid is an endogenous organic acid and an intermediate metabolite derived from leucine catabolism. Impairment of biotin-dependent 3-methylcrotonyl-CoA carboxylase activity leads to accumulation of 3-hydroxyisovaleric acid, making its excretion in urine a sensitive biomarker for biotin deficiency and related metabolic disorders. Accumulation of this metabolite is associated with alterations in cellular energy metabolism and organic aciduria phenotypes. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.