CAS No. 1892595-16-2
Batch:
Purity:99.18
Chemical Structure
Check the
HS148
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 2-[[1-(3-fluorophenyl)-4-oxo-5H-pyrazolo[5,4-d]pyrimidin-6-yl]sulfanyl]butanamide | |
|---|---|---|
| CAS Number | 1892595-16-2 | |
| Molecular Formula |
C15H14FN5O2S |
|
| Molecular Weight (MW) | 347.37 | |
| InChIKey | IQWUQCPIOBQMPK-UHFFFAOYSA-N |
Biological Activity
HS148 is a selective inhibitor of death-associated protein kinase 3 (DAPK3) with a Ki of. By selectively targeting DAPK3 over Pim kinases, HS148 suppresses DAPK3-mediated phosphorylation of smooth muscle myosin light chain and downstream cellular contractile signaling. This compound serves as a valuable molecular probe to differentiate DAPK3-specific signaling from multi-target kinase networks in regulating vascular smooth muscle contractility and hypertension mechanisms. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
2 mg/mL
(5.75 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.