CAS No. 302803-72-1
Batch:
Purity:99.63
Chemical Structure
Check the
HPI-4 (Ciliobrevin A)
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | (E)-3-(2,4-dichlorophenyl)-3-hydroxy-2-(4-oxo-3H-quinazolin-2-yl)prop-2-enenitrile | |
|---|---|---|
| CAS Number | 302803-72-1 | |
| Molecular Formula |
C17H9Cl2N3O2 |
|
| Molecular Weight (MW) | 358.18 | |
| SMILES | C1=CC=C2C(=C1)C(=O)NC(=N2)C(=C(C3=C(C=C(C=C3)Cl)Cl)O)C#N | |
| InChIKey | UEZZGQDPOFILFH-NTCAYCPXSA-N |
HPI-4 (Ciliobrevin A) is a Hedgehog (Hh) pathway antagonist that inhibits Sonic hedgehog (Shh)-induced Hh pathway activation with an IC50 ofdownstream of Smoothened (Smo). By blocking dynein-dependent intraflagellar transport, HPI-4 disrupts primary cilia formation and impairs Gli-mediated transcriptional activity, leading to the repression of downstream Hedgehog-dependent gene expression and signaling phenotypes. [1]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
71 mg/mL
(198.22 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 1 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.