CAS No. 23555-00-2

Batch: Purity:99.73
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Hoechst 34580 Chemical Structure
Chemical Structure
Check the Hoechst 34580 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name N,N-dimethyl-4-[6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazol-2-yl]aniline
CAS Number 23555-00-2
Molecular Formula

C27H29N7
Molecular Weight (MW) 451.57
SMILES CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)N=C(N5)C6=CC=C(C=C6)N(C)C
InChIKey VMCOQLKKSNQANE-UHFFFAOYSA-N

Biological Activity

Hoechst 34580 is a cell-permeable blue fluorescent bis-benzimidazole dye that binds to DNA and acts as an inhibitor of amyloid-beta (Aβ) formation with an IC50 of 0.86 μM. By binding to the minor groove of double-stranded DNA, it enables visualization of nuclear morphology and chromatin condensation during cell cycle and apoptosis assays. Furthermore, its targeted modulation of Aβ processing attenuates toxic protein aggregation and downregulates downstream neurotoxic signaling pathways, highlighting its potential utility in Alzheimer's disease research.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 90 mg/mL
DMSO 5 mg/mL (11.07 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.