CAS No. 23491-52-3

Batch: Purity:99.91
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Hoechst 33342 Chemical Structure
Chemical Structure
Check the Hoechst 33342 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole
CAS Number 23491-52-3
Molecular Formula

C27H28N6O
Molecular Weight (MW) 452.55
SMILES CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C
InChIKey PRDFBSVERLRRMY-UHFFFAOYSA-N

Ordering Information

Biological Activity

Hoechst 33342 is a cell-permeable fluorescent nucleic acid stain that specifically binds to adenine-thymine-rich regions in the minor groove of double-stranded DNA. Upon minor groove binding, the dye exhibits enhanced fluorescence quantum yield, enabling clear visualization of nuclear morphology and chromatin organization in both interphase and mitotic cells. This selective DNA association facilitates live-cell nuclear imaging, cell cycle analysis, and detection of apoptotic nuclear condensation. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 90 mg/mL
DMSO 10 mg/mL (22.09 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.