CAS No. 51-60-5

Batch: Purity:99.91
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Hodostin Chemical Structure
Chemical Structure
Check the Hodostin product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name [3-(dimethylcarbamoyloxy)phenyl]-trimethylazanium;methyl sulfate
CAS Number 51-60-5
Molecular Formula

C13H22N2O6S
Molecular Weight (MW) 334.39
SMILES CN(C)C(=O)OC1=CC=CC(=C1)[N+](C)(C)C.COS(=O)(=O)[O-]
InChIKey OSZNNLWOYWAHSS-UHFFFAOYSA-M

Ordering Information

Biological Activity

Hodostin (Neostigmine methyl sulfate) is a peripherally acting, reversible inhibitor of acetylcholinesterase (AChE) that does not cross the blood-brain barrier. By preventing the degradation of acetylcholine at cholinergic synapses and neuromuscular junctions, it elevates synaptic neurotransmitter levels and enhances cholinergic signaling. This inhibition restores effective neuromuscular impulse transmission and promotes muscular contraction in peripheral tissues.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 67 mg/mL (200.36 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 67 mg/mL
Ethanol 67 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.