CAS No. 156840-13-0
Batch:
Purity:99.75
Chemical Structure
Check the
HBC 530
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 4-[(E)-1-cyano-2-[4-[2-hydroxyethyl(methyl)amino]phenyl]ethenyl]benzonitrile | |
|---|---|---|
| CAS Number | 156840-13-0 | |
| Molecular Formula |
C19H17N3O |
|
| Molecular Weight (MW) | 303.36 | |
| SMILES | CN(CCO)C1=CC=C(C=C1)\C=C(C#N)/C2=CC=C(C=C2)C#N | |
| InChIKey | DIAVZHWDYXQAFC-PDGQHHTCSA-N |
HBCis a green fluorescent protein (GFP) fluorophore mimic that selectively binds to Pepper RNA aptamers. Upon forming a complex with Pepper RNA, its fluorescence is markedly activated, providing high brightness and low background fluorescence for live-cell imaging. This fluorophore-aptamer interaction enables robust visualization and dynamic tracking of cellular RNA species, providing a versatile tool for studying RNA transcription, localization, and cellular dynamics in living cells. [1]
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Concentration
Volume
Mass
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Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
61 mg/mL
(201.08 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.