CAS No. 486-84-0
Batch:
Purity:99.73
Chemical Structure
Check the
Harmane
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 1-methyl-9H-pyrido[3,4-b]indole | |
|---|---|---|
| CAS Number | 486-84-0 | |
| Molecular Formula |
C12H10N2 |
|
| Molecular Weight (MW) | 182.22 | |
| SMILES | CC1=NC=CC2=C1[NH]C3=CC=CC=C23 | |
| InChIKey | PSFDQSOCUJVVGF-UHFFFAOYSA-N |
Biological Activity
Harmane is a selective monoamine oxidase inhibitor that targets human MAO-A and human MAO-B (IC50 5 μM). Additionally, harmane inhibits the apurinic/apyrimidinic (AP) endonuclease activity of phage T4-induced UV endonuclease. By blunting monoamine oxidase activity, harmane reduces the oxidative deamination of monoamine neurotransmitters, thereby modulating central neurotransmission pathways and associated cellular and neurological phenotypes. [1][2]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
36 mg/mL
(197.56 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 36 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
Jump to
Note: Technical data last updated: Sep 1, 2026.