Harmaline

Catalog No.S4776

For research use only.

Harmaline, a natural occurrence β-carboline alkaloid, is a central nervous system stimulant and an acetylcholinesterase (AChR) inhibitor; also inhibits histamine N-methyltransferase.

Harmaline Chemical Structure

CAS No. 304-21-2

Purity & Quality Control

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Biological Activity

Description Harmaline, a natural occurrence β-carboline alkaloid, is a central nervous system stimulant and an acetylcholinesterase (AChR) inhibitor; also inhibits histamine N-methyltransferase.
In vitro

Harmaline (HAR) could inhibit tumor cell proliferation and induce G2/M cell cycle arrest accompanied by an increase in apoptotic cell death in SGC-7901 cancer cells. In SGC-7901 cells, exposure to harmaline for 48 h results in morphologic characteristic alterations of apoptosis, including membrane blebbing, nuclear condensation and granular apoptotic bodie. Harmaline could up-regulate the expressions of cell cycle-related proteins of p-Cdc2, p21, p-p53, Cyclin B and down-regulate the expression of p-Cdc25C. In addition, Harmaline could up-regulate the expressions of Fas/FasL, activate Caspase-8 and Caspase-3[1].

In vivo Harmaline could exert on antitumor activity with a dose of 15 mg/kg/day in vivo, which is related with cell cycle arrest[1]. Harmaline also induces cognitive disturbances such as motor and spatial learning impairments. Lower doses of harmaline (5–10 mg/kg) have anxiogenic effects since higher doses (20 mg/kg) have anxiolytic-like properties. Harmaline acts on emotional reactivity in mice by influencing their decision making when placed in an anxiogenic situation[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: SGC-7901 cells
  • Concentrations: 0-10 μM
  • Incubation Time: 48 h
  • Method: SGC-7901 cells are treated with different concentrations of HAR (0-10 μM) for 48 h. Cell viability is then measured using MTT method.
Animal Research:[1]
  • Animal Models: Healthy male nude mice (Balb/c, 6–8 weeks of age)
  • Dosages: 5, 15, 30 mg/kg/day
  • Administration: oral administration

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 214.26
Formula

C13H14N2O

CAS No. 304-21-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=NCCC2=C1NC3=C2C=CC(=C3)OC

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