CAS No. 2253733-10-5
Batch:
Purity:99.42
Chemical Structure
Check the
HADA hydrochloride
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | (2R)-2-amino-3-[(7-hydroxy-2-oxochromene-3-carbonyl)amino]propanoic acid;hydrochloride | |
|---|---|---|
| CAS Number | 2253733-10-5 | |
| Molecular Formula |
C13H12N2O6.ClH |
|
| Molecular Weight (MW) | 328.71 | |
| InChIKey | PLVOEKQNBIXHDI-SBSPUUFOSA-N |
Biological Activity
HADA hydrochloride (HCC-amino-D-alanine hydrochloride) is a blue fluorescent D-amino acid probe that labels bacterial peptidoglycans in live cells without affecting cell growth. By substituting for standard bacterial cell wall precursors, it incorporates into nascent peptidoglycan chains via endogenous transpeptidase activity. This metabolic integration enables real-time spatiotemporal visualization of cell wall synthesis, active growth zones, and septal division sites across diverse bacterial species while exhibiting mitochondrial outer membrane permeability.[1][2]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
66 mg/mL
(200.78 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
Jump to
Note: Technical data last updated: Sep 1, 2026.