CAS No. 4091-99-0

Batch: Purity:99.93
Check CoA
H2DCFDA (DCFH-DA) Chemical Structure
Chemical Structure
Check the H2DCFDA (DCFH-DA) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
CAS Number 4091-99-0
Molecular Formula

C24H16Cl2O7
Molecular Weight (MW) 487.29
SMILES CC(=O)OC1=CC2=C(C=C1Cl)C(C3=C(O2)C=C(OC(C)=O)C(=C3)Cl)C4=C(C=CC=C4)C(O)=O
InChIKey PXEZTIWVRVSYOK-UHFFFAOYSA-N

Biological Activity

H2DCFDA (DCFH-DA) is a cell-permeable fluorogenic probe widely utilized to detect intracellular reactive oxygen species (ROS) production. Upon entering live cells, non-fluorescent H2DCFDA is cleaved by intracellular esterases into 2′,7′-dichlorofluorescin, which is subsequently oxidized by ROS into the highly fluorescent compound 2′,7′-dichlorofluorescein (Ex/Em = 488/. This intracellular conversion enables sensitive, quantitative assessment of cellular oxidative stress and oxidative metabolism in phagocytes and other cell types. [1][2]

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 97 mg/mL (199.06 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 7 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Wang H, et al. Free Radic Biol Med. 1999;27(5-6):683-94.

[2] Gomes A, et al. J Biochem Biophys Methods. 2005;65(2-3):45-80.

Jump to

Note: Technical data last updated: Sep 1, 2026.