CAS No. 593-84-0
Batch:
Purity:100.00
Chemical Structure
Check the
Guanidine thiocyanate
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| CAS Number | 593-84-0 | |
|---|---|---|
| Molecular Formula |
CH5N3.CHNS |
|
| Molecular Weight (MW) | 118.16 |
Guanidine thiocyanate is a chaotropic agent and potent protein denaturant that acts as a strong inhibitor of ribonucleases and other nucleases. By disrupting hydrophobic interactions and hydrogen bonding within proteins, it inactivates RNase enzymes to prevent enzymatic degradation of RNA during extraction procedures. This protein-denaturing activity also deactivates high-titer viruses in biological samples, ensuring the stability, purity, and integrity of isolated nucleic acids. [1]
|
Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
24 mg/mL
(203.11 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 24 mg/mL |
| Ethanol | 24 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.