CAS No. 1262041-49-5

Batch: Purity:99.93
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GS-444217 Chemical Structure
Chemical Structure
Check the GS-444217 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 4-(4-cyclopropylimidazol-1-yl)-N-[3-(4-cyclopropyl-1,2,4-triazol-3-yl)phenyl]pyridine-2-carboxamide
CAS Number 1262041-49-5
Molecular Formula

C23H21N7O
Molecular Weight (MW) 411.46
SMILES C1CC1C2=CN(C=N2)C3=CC(=NC=C3)C(=O)NC4=CC=CC(=C4)C5=NN=CN5C6CC6
InChIKey ZGCMQKWOUIMBEP-UHFFFAOYSA-N

Biological Activity

GS-444217 is a potent, ATP-competitive, selective inhibitor targeting apoptosis signal-regulating kinase 1 (ASK1). By directly inhibiting ASK1 kinase activity, the compound prevents the phosphorylation and downstream activation of the JNK and p38 MAPK signaling cascades. This targeted inhibition effectively blocks oxidative stress-mediated pro-inflammatory cytokine production and suppresses cellular apoptotic responses across various cell-based experimental models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 60 mg/mL (145.82 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Ichijo H, et al. Science. 1997;275(5296):90-4.

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Note: Technical data last updated: Sep 1, 2026.