CAS No. 2374856-75-2
Batch:
Purity:99.08
Chemical Structure
Check the
GS-4224
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | (5S)-5-[[[5-[2-chloro-3-[2-chloro-3-[6-methoxy-5-[[[(2S)-5-oxopyrrolidin-2-yl]methylamino]methyl]pyrazin-2-yl]phenyl]phenyl]-3-methoxypyrazin-2-yl]methylamino]methyl]pyrrolidin-2-one | |
|---|---|---|
| CAS Number | 2374856-75-2 | |
| Molecular Formula |
C34H36Cl2N8O4 |
|
| Molecular Weight (MW) | 691.61 | |
| InChIKey | OIIOPWHTJZYKIL-PMACEKPBSA-N |
Biological Activity
GS-4224 (evixapodlin) is an orally bioavailable small-molecule inhibitor of the human programmed cell death 1/programmed cell death ligand 1 (PD-1/PD-L1) interaction. Binding of GS-4224 induces dimerization of cell-surface PD-L1, which selectively blocks its engagement with PD-1 and restores downstream T-cell receptor signaling. Reversal of this inhibitory checkpoint pathway reactivates cytotoxic T lymphocytes, promoting increased tumor cell killing both in vitro and in vivo. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
100 mg/mL
(144.59 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 12 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.