CAS No. 1032754-81-6

Batch: Purity:99.93
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GNE-477 Chemical Structure
Chemical Structure
Check the GNE-477 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 5-[7-methyl-6-[(4-methylsulfonylpiperazin-1-yl)methyl]-4-morpholin-4-ylthieno[3,2-d]pyrimidin-2-yl]pyrimidin-2-amine
CAS Number 1032754-81-6
Molecular Formula

C21H28N8O3S2
Molecular Weight (MW) 504.63
SMILES CC1=C(SC2=C1N=C(N=C2N3CCOCC3)C4=CN=C(N=C4)N)CN5CCN(CC5)S(=O)(=O)C
InChIKey AKKCGLXULFRAET-UHFFFAOYSA-N

Ordering Information

Biological Activity

GNE-477 is a potent dual PI3K/mTOR inhibitor targeting PI3Kα and mTOR (Kiapp. By inhibiting both PI3Kα and mTOR kinase activities, it potently downregulates downstream AKT/mTOR signaling pathways. This inhibition leads to the suppression of cellular proliferation and the induction of apoptosis in human cancer cells, demonstrating robust preclinical antitumor efficacy.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 25 mg/mL (49.54 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.