CAS No. 108321-42-2

Batch: Purity:97
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G418 Sulfate (Geneticin) Chemical Structure
Chemical Structure
Check the G418 Sulfate (Geneticin) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (2S,3S,4S,5S)-2-[(1S,2S,3R,4S,6R)-4,6-diamino-3-[(2S,3R,4R,5S,6R)-3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid
CAS Number 108321-42-2
Molecular Formula

C20H40N4O10.2H2SO4
Molecular Weight (MW) 692.71
SMILES CC(C1C(C(C(C(O1)OC2C(CC(C(C2O)OC3C(C(C(CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O
InChIKey UHEPSJJJMTWUCP-NKCAIAFTSA-N

Biological Activity

Geneticin (G418 Sulfate) is an aminoglycoside antibiotic that acts as an elongation inhibitor of 80S ribosomes. By binding to the ribosomal decoding site, Geneticin disrupts polypeptide chain elongation during translation in both eukaryotic and prokaryotic cells. This broad inhibition of protein synthesis halts cellular growth and induces cell death, making it widely utilized as a selection agent for screening transfected or transformed cells. [1][2]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 100 mg/mL
DMSO Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Reference

[1] Eustice DC, et al. Antimicrob Agents Chemother. 1984;26(1):53-60.

[2] Vicens Q, et al. J Mol Biol. 2003;326(4):1175-88.

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Note: Technical data last updated: Sep 1, 2026.