CAS No. 121577-32-0

Batch: Purity:99.96
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Gatifloxacin hydrochloride Chemical Structure
Chemical Structure
Check the Gatifloxacin hydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-cyclopropyl-6-fluoro-8-methoxy-7-(3-methylpiperazin-1-yl)-4-oxoquinoline-3-carboxylic acid;hydrochloride
CAS Number 121577-32-0
Molecular Formula

C19H23ClFN3O4
Molecular Weight (MW) 411.86
SMILES Cl.COC1=C2N(C=C(C(O)=O)C(=O)C2=CC(=C1N3CCNC(C)C3)F)C4CC4
InChIKey GQYBNVXJQVIRGC-UHFFFAOYSA-N

Biological Activity

Gatifloxacin hydrochloride is a broad-spectrum fluoroquinolone antibiotic that targets bacterial type II topoisomerases, inhibiting E. coli DNA gyrase (IC50 0.109 μg/mL) and S. aureus topoisomerase IV (IC50 13.8 μg/mL). By blocking DNA gyrase and topoisomerase IV, it disrupts bacterial DNA replication and transcription, ultimately leading to bacterial cell death and broad antibacterial activity against Gram-positive and Gram-negative pathogens. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 20 mg/mL
DMSO 4 mg/mL (9.71 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Takei M, et al. Antimicrob Agents Chemother. 1998;42(10):2678-81.

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Note: Technical data last updated: Sep 1, 2026.