CAS No. 1394238-91-5

Batch: Purity:98.03
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Fmoc-Val-Ala-PAB Chemical Structure
Chemical Structure
Check the Fmoc-Val-Ala-PAB product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 1394238-91-5
Molecular Formula

C30H33N3O5
Molecular Weight (MW) 515.60
SMILES CC(C)C(C(=O)NC(C)C(=O)NC1=CC=C(C=C1)CO)NC(=O)OCC2C3=CC=CC=C3C4=CC=CC=C24

Biological Activity

Fmoc-Val-Ala-PAB is a cathepsin B-cleavable dipeptide linker used in the construction of antibody-drug conjugates (ADCs) for targeted drug delivery. Upon receptor-mediated endocytosis and lysosomal trafficking, the valine-alanine sequence undergoes enzymatic cleavage by lysosomal cathepsin B, promoting intracellular release of the conjugated cytotoxic payload. This targeted cleavage mechanism enhances drug release within tumor cells while maintaining stability in circulation to induce targeted cytotoxicity and tumor regression. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (193.94 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Dubowchik GM, et al. Bioconjug Chem. 2002;13(4):855-69.

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Note: Technical data last updated: Sep 1, 2026.