CAS No. 2412089-96-2

Batch: Purity:99.04
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Fluorofurimazine (FFz) Chemical Structure
Chemical Structure
Check the Fluorofurimazine (FFz) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 6-(3-amino-2-fluorophenyl)-8-[(2-fluorophenyl)methyl]-2-(furan-2-ylmethyl)imidazo[1,2-a]pyrazin-3-ol
CAS Number 2412089-96-2
Molecular Formula

C24H18F2N4O2
Molecular Weight (MW) 432.42
InChIKey ZCOXIHLUZKFAPD-UHFFFAOYSA-N

Biological Activity

Fluorofurimazine is a fluorinated furimazine derivative that functions as a bioluminescent substrate for NanoLuc luciferase and engineered NanoLuc variants such as Antares. Upon oxidation by NanoLuc, fluorofurimazine yields high signal brightness and enhanced aqueous solubility compared to furimazine, facilitating improved optical bioavailability in vivo. This substrate enables non-invasive, high-sensitivity bioluminescence imaging to monitor biological processes and cell dynamics in animal models. [1]

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 86 mg/mL (198.88 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Su Y, et al. Nat Methods. 2020;17(8):852-860.

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Note: Technical data last updated: Sep 1, 2026.