CAS No. 4404-43-7
Batch:
Purity:99.00
Chemical Structure
Check the
Fluorescent Brightener 28
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| CAS Number | 4404-43-7 | |
|---|---|---|
| Molecular Formula |
C40H44N12O10S2 |
|
| Molecular Weight (MW) | 916.99 |
Biological Activity
Fluorescent Brightener 28 is a fluorescent probe and cell-wall staining dye that selectively binds to β-1,3 and β-1,4 glucans, cellulose, and chitin. Upon binding to chitin structures in fungal cell walls, it fluoresces intensely under ultraviolet light, facilitating the identification and visualization of fungi, yeast, and plant cell wall architecture without interfering with cell viability. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
5 mg/mL
(5.45 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.) |
| Water | 4 mg/mL |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.