CAS No. 1995893-58-7

Batch: Purity:99.64
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FEN1-IN-4 Chemical Structure
Chemical Structure
Check the FEN1-IN-4 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-(cyclopropylmethyl)-3-hydroxyquinazoline-2,4-dione
CAS Number 1995893-58-7
Molecular Formula
C12H12N2O3
Molecular Weight (MW) 232.24
SMILES ON1C(=O)N(CC2CC2)C3=C(C=CC=C3)C1=O
InChIKey JAFLWTUYSKADJS-UHFFFAOYSA-N

Biological Activity

FEN1-IN-4 is a potent inhibitor of human flap endonuclease-1 (hFEN1). By targeting hFEN1, it selectively disrupts critical endonucleolytic cleavage during Okazaki fragment processing and base excision repair pathways. Blockade of hFEN1 activity causes the accumulation of unhandled DNA intermediates, impairing cellular DNA repair mechanisms and promoting genomic instability in compromised cells. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 46 mg/mL (198.07 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 46 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Exell JC, et al. Nat Chem Biol. 2016;12(10):815-21.

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Note: Technical data last updated: Sep 1, 2026.