CAS No. 824983-91-7

Batch: Purity:96.29
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FEN1-IN-1 Chemical Structure
Chemical Structure
Check the FEN1-IN-1 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-(2,3-dihydro-1,4-benzodioxin-3-ylmethyl)-3-hydroxythieno[3,2-d]pyrimidine-2,4-dione
CAS Number 824983-91-7
Molecular Formula

C15H12N2O5S
Molecular Weight (MW) 332.33
InChIKey MXQGCMQXTPTJJT-UHFFFAOYSA-N

Biological Activity

FEN1-IN-1 is an inhibitor of flap endonuclease 1 (FEN1) that binds directly to the active site and suppresses enzymatic activity in part through coordination with magnesium ions. Blocking FEN1 function initiates a cellular DNA damage response, leading to downstream activation of the ATM checkpoint pathway, histone H2AX phosphorylation, and FANCD2 ubiquitination in mammalian cells. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 66 mg/mL (198.59 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Balakrishnan L, et al. Annu Rev Biochem. 2013;82:119-38.

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Note: Technical data last updated: Sep 1, 2026.