CAS No. 314-13-6

Batch: Purity:99.05
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Evans Blue Chemical Structure
Chemical Structure
Check the Evans Blue product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 314-13-6
Molecular Formula

C34H24N6O14S4.4Na
Molecular Weight (MW) 960.81
SMILES CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C3)C(=CC(=C4N)S(=O)(=O)[O-])S(=O)(=O)[O-])O)C)N=NC5=C(C6=C(C=C5)C(=CC(=C6N)S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+].[Na+].[Na+].[Na+]

Biological Activity

Evans Blue is a potent inhibitor of L-glutamate uptake into synaptic vesicles and acts as an AMPA/kainate receptor antagonist. By blocking vesicular glutamate transport, it disrupts excitatory neurotransmission and reduces glutamatergic signaling in neuronal pathways. In addition, its antagonism of AMPA and kainate receptors modulates postsynaptic ionotropic glutamate receptor activity, influencing synaptic plasticity and neuronal excitability. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (104.07 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 100 mg/mL
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Roseth S, et al. J Neurochem. 1995;65(1):96-103.

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Note: Technical data last updated: Sep 1, 2026.