CAS No. 16423-68-0
Batch:
Purity:99.89
Chemical Structure
Check the
Erythrosine B
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 3',6'-dihydroxy-2',4',5',7'-tetraiodospiro[2-benzofuran-3,9'-xanthene]-1-one | |
|---|---|---|
| CAS Number | 16423-68-0 | |
| Molecular Formula |
C20H6I4Na2O5 |
|
| Molecular Weight (MW) | 879.86 | |
| SMILES | C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C(=C4OC5=C(C(=C(C=C35)I)[O-])I)I)[O-])I.[Na+].[Na+] | |
| InChIKey | OALHHIHQOFIMEF-UHFFFAOYSA-N |
Biological Activity
Erythrosine B is a synthetic dye that acts as a potent inhibitor of plasma membrane Ca2+-ATPase (PMCA). By inhibiting PMCA activity, it impairs active calcium transport across cell membranes, disrupting intracellular calcium homeostasis. This alteration in cytosolic calcium levels affects calcium-dependent signaling cascades and cellular physiological functions, making it a valuable tool compound for studying calcium transport mechanisms.
How to Prepare Stock Solution?
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Concentration
Volume
Mass
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Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
100 mg/mL
(113.65 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
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Note: Technical data last updated: Sep 1, 2026.