CAS No. 3521-62-8

Batch: Purity:99.64
Erythromycin estolate Chemical Structure
Chemical Structure
Check the Erythromycin estolate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 3521-62-8
Molecular Formula

C52H97NO18S

Molecular Weight (MW) 1056.39
SMILES CCCCCCCCCCCCOS(=O)(=O)O.CCC1C(C(C(C(=O)C(CC(C(C(C(C(C(=O)O1)C)OC2CC(C(C(O2)C)O)(C)OC)C)OC3C(C(CC(O3)C)N(C)C)OC(=O)CC)(C)O)C)C)O)(C)O

Ordering Information

Biological Activity

Erythromycin estolate is a macrolide antibiotic that inhibits bacterial protein synthesis by reversibly binding to the 50S ribosomal subunit. By blocking the peptidyl transferase center and interfering with aminoacyl translocation, erythromycin halts nascent polypeptide chain elongation. This mechanism impairs bacterial ribosome function and arrests bacterial growth, demonstrating potent antibacterial activity against Gram-positive and select Gram-negative organisms. [1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (94.66 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.