CAS No. 265646-85-3

Batch: Purity:99.71
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Endovion (NS 3728) Chemical Structure
Chemical Structure
Check the Endovion (NS 3728) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-[3,5-bis(trifluoromethyl)phenyl]-3-[5-bromo-2-(2H-tetrazol-5-yl)phenyl]urea
CAS Number 265646-85-3
Molecular Formula

C16H9BrF6N6O
Molecular Weight (MW) 495.18
SMILES FC(F)(F)C1=CC(=CC(=C1)NC(=O)NC2=CC=C(Br)C=C2C3=NN=N[NH]3)C(F)(F)F
InChIKey OQRAKHDEGGGWQO-UHFFFAOYSA-N

Ordering Information

Biological Activity

Endovion (NS 3728) is an acidic di-aryl-urea anion channel inhibitor that blocks volume-regulated anion channels (VRAC) with an IC50 of 0.40 µM. Inhibition of VRAC disrupts osmotic swelling-activated chloride conductance and regulatory volume decrease. This blockade interferes with essential downstream cell-volume signaling pathways, resulting in suppressed cell proliferation, altered apoptotic volume regulation, and reduced cell migration.[1][2]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (201.94 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.