CAS No. 496807-64-8
Batch:
Purity:99.67
Chemical Structure
Check the
EN460
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 2-chloro-5-[(4Z)-5-oxo-4-[(5-phenylfuran-2-yl)methylidene]-3-(trifluoromethyl)pyrazol-1-yl]benzoic acid | |
|---|---|---|
| CAS Number | 496807-64-8 | |
| Molecular Formula |
C22H12ClF3N2O4 |
|
| Molecular Weight (MW) | 460.79 | |
| InChIKey | RSLFQCNAOMQAIH-WJDWOHSUSA-N |
Biological Activity
EN460 is a small-molecule inhibitor of endoplasmic reticulum oxidation 1 (ERO1) that selectively targets the reduced active form of ERO1α to prevent its re-oxidation. By inhibiting ERO1α-mediated oxidative protein folding within the endoplasmic reticulum, EN460 triggers signaling through the unfolded protein response pathway, thereby preconditioning cells against severe endoplasmic reticulum stress and protecting cells from erastin-induced ferroptosis. [1]
How to Prepare Stock Solution?
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Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
17 mg/mL
(36.89 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.