CAS No. 793719-01-4
Batch:
Purity:99.00
Chemical Structure
Check the
EN450
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | N-[2-chloro-5-(dimethylsulfamoyl)phenyl]prop-2-enamide | |
|---|---|---|
| CAS Number | 793719-01-4 | |
| Molecular Formula |
C11H13ClN2O3S |
|
| Molecular Weight (MW) | 288.75 | |
| InChIKey | OXFXDOLAQBMOPH-UHFFFAOYSA-N |
EN450 is a cysteine-reactive covalent molecular glue degrader targeting the transcription factor NF-κB. It covalently engages allosteric C111 of the E2 ubiquitin-conjugating enzyme UBE2D to induce ternary complex formation between UBE2D and NFKB1. This targeted recruitment promotes Cullin E3 ligase- and proteasome-dependent degradation of NFKB1, disrupting NF-κB signaling pathways and producing potent anti-proliferative effects in cancer cells.
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
58 mg/mL
(200.86 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 15 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.