CAS No. 1675821-32-5
Batch:
Purity:99.49
Chemical Structure
Check the
EML 425
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 1,3-dibenzyl-5-[(4-hydroxy-2,6-dimethylphenyl)methylidene]-1,3-diazinane-2,4,6-trione | |
|---|---|---|
| CAS Number | 1675821-32-5 | |
| Molecular Formula |
C27H24N2O4 |
|
| Molecular Weight (MW) | 440.49 | |
| SMILES | CC1=C(C=C2C(=O)N(CC3=CC=CC=C3)C(=O)N(CC4=CC=CC=C4)C2=O)C(=CC(=C1)O)C | |
| InChIKey | LUGQBJDYUPNAQQ-UHFFFAOYSA-N |
Biological Activity
EML 425 is a potent and selective histone acetyltransferase inhibitor targeting CREB binding protein (CBP, IC50 = and p300 (IC50 =. By targeting the catalytic domain of CBP/p300, EML 425 suppresses cellular histone acetylation and disrupts key transcriptional pathways. This blockade downstream leads to inhibition of cellular proliferation, cell cycle arrest, and induction of apoptosis in human leukemic cell lines. [1]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
88 mg/mL
(199.77 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Ethanol | 2 mg/mL |
| Water | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
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Note: Technical data last updated: Sep 1, 2026.