CAS No. 67-42-5
Batch:
Purity:98.09
Chemical Structure
Check the
EGTA
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 2-[2-[2-[2-[bis(carboxymethyl)amino]ethoxy]ethoxy]ethyl-(carboxymethyl)amino]acetic acid | |
|---|---|---|
| CAS Number | 67-42-5 | |
| Molecular Formula |
C14H24N2O10 |
|
| Molecular Weight (MW) | 380.35 | |
| InChIKey | DEFVIWRASFVYLL-UHFFFAOYSA-N |
EGTA is a specific calcium ion chelator that demonstrates high selectivity for calcium ions over magnesium ions. By effectively sequestering free intracellular and extracellular calcium ions, EGTA disrupts calcium-dependent signaling pathways. This depletion impairs calcium-activated enzymatic processes, membrane transport, cell-cell adhesion, and excitation-contraction coupling across diverse biological systems and cell models. [1]
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Concentration
Volume
Mass
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Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.