CAS No. 25952-53-8
Batch:
Purity:99.09
Chemical Structure
Check the
EDC hydrochloride
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| CAS Number | 25952-53-8 | |
|---|---|---|
| Molecular Formula |
C8H17N3.ClH |
|
| Molecular Weight (MW) | 191.7 | |
| SMILES | Cl.CCN=C=NCCCN(C)C |
EDC hydrochloride is a water-soluble zero-length carbodiimide crosslinking reagent that activates carboxyl groups to form reactive intermediates for condensation with primary amines or nucleic acid modification. By facilitating direct covalent amide bond formation without adding extra spacer atoms, EDC stabilizes noncovalent protein complexes and biomolecular conjugates. This zero-length crosslinking mechanism maintains molecular assemblies for structural analysis and functional characterization of macromolecular interactions.[1][2]
|
Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
38 mg/mL
(198.22 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 38 mg/mL |
| Ethanol | 38 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.