CAS No. 229476-53-3

Batch: Purity:99.85
EBE-A22 Chemical Structure
Chemical Structure
Check the EBE-A22 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 229476-53-3
Molecular Formula

C17H16BrN3O2

Molecular Weight (MW) 374.23
SMILES CN(C1=CC(=CC=C1)Br)C2=NC=NC3=CC(=C(C=C32)OC)OC

Ordering Information

Biological Activity

EBE-A22 is an inactive derivative of the EGFR (ErbB-1) tyrosine kinase inhibitor PD 153035. Unlike its parent compound, which potently blocks ErbB-1 receptor autophosphorylation and suppresses downstream EGFR-mediated signaling pathways, EBE-A22 lacks inhibitory activity against ErbB-1 phosphorylation. Consequently, EBE-A22 serves as a negative control compound in cellular and biochemical studies evaluating EGFR-dependent signaling processes and phenotypic responses. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 9 mg/mL (24.04 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.