CAS No. 924296-18-4

Batch: Purity:99.86
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Dubs-IN-1 Chemical Structure
Chemical Structure
Check the Dubs-IN-1 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name (9E)-9-phenylmethoxyiminoindeno[1,2-b]pyrazine-2,3-dicarbonitrile
CAS Number 924296-18-4
Molecular Formula

C20H11N5O 
Molecular Weight (MW) 337.33
InChIKey GKOWDIBLCDZJHF-NCELDCMTSA-N

Biological Activity

Dubs-IN-1 is a small-molecule inhibitor of ubiquitin-specific proteases (USPs), targeting USPwith an ICof. By blocking USPdeubiquitinase activity, Dubs-IN-1 disrupts ubiquitin-mediated protein stability and endosomal trafficking pathways, leading to increased protein turnover. Consequently, this targeted inhibition suppresses cancer cell viability and attenuates tumor cell proliferation across multiple cell lines.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 17 mg/mL (50.39 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.