CAS No. 68528-80-3
Batch:
Purity:97
Chemical Structure
Check the
DSS Crosslinker
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | bis(2,5-dioxopyrrolidin-1-yl) octanedioate | |
|---|---|---|
| CAS Number | 68528-80-3 | |
| Molecular Formula |
C16H20N2O8 |
|
| Molecular Weight (MW) | 368.34 | |
| SMILES | C1CC(=O)N(C1=O)OC(=O)CCCCCCC(=O)ON2C(=O)CCC2=O | |
| InChIKey | ZWIBGKZDAWNIFC-UHFFFAOYSA-N |
DSS Crosslinker (Disuccinimidyl suberate) is a membrane-permeable, homobifunctional amine-reactive crosslinking agent and non-cleavable ADC linker. It contains N-hydroxysuccinimide ester groups at both ends that target primary amines, such as lysine side chains, to form stable covalent amide bonds. This crosslinker enables covalent coupling of proteins and stabilization of protein complexes for structural analysis and antibody-drug conjugate preparation. [1]
|
Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
74 mg/mL
(200.9 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
| DMSO |
74 mg/mL
(200.9 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.