Cell-free Assays (IC50/Ki) for Doxorubicin (Adriamycin) Hydrochloride

Key TargetIC50 (Cell-free)DescriptionReference
Mtb DnaG 7.7 ± 0.5 mM Coupled colorimetric primase–pyrophosphatase assay, dose–response equation fitting with SigmaPlot 9.0 PMC3575809

Cell-based Assay (IC50) for Doxorubicin (Adriamycin) Hydrochloride

Cell TypeIC50 (Cell-based)DescriptionReference
MES-SA 31.6 nM PrestoBlue viability reagent, Sigmoidal curve fitting Download PDF
MES-SA/Dx5 3.16 µM PrestoBlue viability reagent, Sigmoidal curve fitting Download PDF
HeLa cell line 1.0±0.2 μM crystal violet assay PMC6235738
MCF-7 2.10 µM Cell Counting Kit-8 (CCK-8) assay PMC12919367
MCF-7 1.45 µM Cell Counting Kit-8 (CCK-8) assay PMC12919367
Check the Doxorubicin (Adriamycin) Hydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

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