CAS No. 23674-86-4

Batch: Purity:99.96
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Difluprednate Chemical Structure
Chemical Structure
Check the Difluprednate product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 23674-86-4
Molecular Formula

C27H34F2O7
Molecular Weight (MW) 508.55
SMILES CCCC(=O)OC1(CCC2C1(CC(C3(C2CC(C4=CC(=O)C=CC43C)F)F)O)C)C(=O)COC(=O)C

Ordering Information

Biological Activity

Difluprednate is a synthetic difluorinated corticosteroid derivative that functions as a potent glucocorticoid receptor agonist. Upon binding to the glucocorticoid receptor, it modulates inflammatory signaling cascades and suppresses the release of pro-inflammatory cytokines, mediators, and arachidonic acid metabolites. This mechanism leads to significant inhibition of ocular and cutaneous inflammatory responses, vascular permeability, and tissue edema in preclinical inflammation models. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (196.63 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol 100 mg/mL
Water Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.