CAS No. 28718-90-3
Batch:
Purity:99.9
Chemical Structure
Check the
DAPI Dihydrochloride
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
Chemical Information
| IUPAC Name | 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide;dihydrochloride | |
|---|---|---|
| CAS Number | 28718-90-3 | |
| Molecular Formula |
C16H15N5.2ClH |
|
| Molecular Weight (MW) | 350.25 | |
| SMILES | Cl.Cl.NC(=N)C1=CC=C(C=C1)C2=CC3=CC=C(C=C3[NH]2)C(N)=N | |
| InChIKey | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
Biological Activity
DAPI Dihydrochloride is a fluorescent nuclear stain that preferentially binds to adenine-thymine (A-T) rich regions of double-stranded DNA. Upon binding to the minor groove of DNA, the dye exhibits a significant enhancement in fluorescence intensity, enabling accurate visualization and quantification of nuclear morphology and chromatin structure. This selective interaction allows effective detection of nuclear DNA changes during cell cycle analysis, apoptosis, and cellular staining procedures. [1][2]
How to Prepare Stock Solution?
|
Concentration
Volume
Mass
|
|---|
Solubility
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
70 mg/mL
(199.85 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 10 mg/mL |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Reference
Jump to
Note: Technical data last updated: Sep 1, 2026.