CAS No. 103404-75-7

Batch: Purity:99.92
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D-Luciferin sodium salt Chemical Structure
Chemical Structure
Check the D-Luciferin sodium salt product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name sodium (4S)-2-(6-hydroxy-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylate
CAS Number 103404-75-7
Molecular Formula
C11H7N2NaO3S2
Molecular Weight (MW) 302.3
SMILES OC1=CC=C2N=C(SC2=C1)C3=NC(CS3)C(=O)O[Na]
InChIKey LILQLBIQROYWIA-OGFXRTJISA-M

Biological Activity

D-Luciferin sodium salt is a natural substrate for firefly luciferase that enables bioluminescent signal generation. In the presence of ATP, magnesium, and oxygen, firefly luciferase oxidizes D-luciferin to oxyluciferin, yielding light emission. This ATP-dependent enzymatic reaction is widely utilized in bioluminescence imaging, reporter gene assays, cell viability monitoring, and real-time tracking of cellular processes in vitro and in vivo. [1]

Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
Water 100 mg/mL
DMSO 40 mg/mL (132.31 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Contag CH, et al. Annu Rev Biomed Eng. 2002;4:235-60.

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Note: Technical data last updated: Sep 1, 2026.