CAS No. 5870-29-1

Batch: Purity:99.95
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Cyclopentolate Hydrochloride Chemical Structure
Chemical Structure
Check the Cyclopentolate Hydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 5870-29-1
Molecular Formula

C17H26ClNO3
Molecular Weight (MW) 327.85
SMILES CN(C)CCOC(=O)C(C1=CC=CC=C1)C2(CCCC2)O.Cl

Biological Activity

Cyclopentolate Hydrochloride is an antagonist of muscarinic acetylcholine receptors, specifically targeting M1, M2, and M3 receptor subtypes. By competitively blocking acetylcholine binding to muscarinic receptors, it disrupts G protein-coupled receptor signaling pathways, including phospholipase C activation and intracellular calcium mobilization. This inhibition blocks parasympathetic cholinergic responses, leading to smooth muscle relaxation and downstream phenotypic effects such as pupil dilation and accommodation paralysis. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 66 mg/mL (201.31 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 66 mg/mL
Ethanol 66 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Caulfield MP, et al. Pharmacol Rev. 1998;50(2):279-90.

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Note: Technical data last updated: Sep 1, 2026.