CAS No. 146368-11-8
Batch:
Purity:99.77
Chemical Structure
Check the
CY5
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | (2E)-2-[(2E,4E)-5-[1-(5-carboxypentyl)-3,3-dimethyl-5-sulfoindol-1-ium-2-yl]penta-2,4-dienylidene]-1-ethyl-3,3-dimethylindole-5-sulfonate | |
|---|---|---|
| CAS Number | 146368-11-8 | |
| Molecular Formula |
C33H40N2O8S2 |
|
| Molecular Weight (MW) | 656.81 | |
| InChIKey | HPICMEGAGMPYID-UHFFFAOYSA-N |
CY5 (Sulfo-Cyanine5) is a far-red cyanine fluorescent dye widely used for labeling nucleic acids, proteins, and other biomolecules. Functioning as an acceptor fluorophore in Förster resonance energy transfer (FRET) assays, CY5 enables single-molecule tracking of structural conformational dynamics, RNA folding pathways, and intermolecular interactions. Structural analysis demonstrates that terminally attached CY5 stacks onto double-stranded DNA duplexes to facilitate accurate distance determinations in single-molecule FRET applications.[1][2]
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Concentration
Volume
Mass
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Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
100 mg/mL
(152.25 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 25 mg/mL |
| Ethanol | 8 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.