CAS No. 53518-15-3
Batch:
Purity:99.69
Chemical Structure
Check the
Coumarin 151
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| CAS Number | 53518-15-3 | |
|---|---|---|
| Molecular Formula |
C10H6F3NO2 |
|
| Molecular Weight (MW) | 229.16 | |
| SMILES | C1=CC2=C(C=C1N)OC(=O)C=C2C(F)(F)F |
Coumarin 151 (7-Amino-4-(trifluoromethyl)coumarin) is a fluorogenic indicator and fluorescent tracer widely utilized for the detection and quantification of proteinase activities. Upon enzymatic cleavage of peptide substrates conjugated to this coumarin derivative, the released fluorophore exhibits strong fluorescence, enabling sensitive real-time monitoring of proteolytic cleavage in various biochemical assays. This fluorescent signaling strategy facilitates the characterization of protease kinetics, substrate specificity, and enzyme inhibition profiles across diverse enzymatic pathways.
|
Concentration
Volume
Mass
|
1 mg | 5 mg | 10 mg |
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
46 mg/mL
(200.73 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.