CAS No. 1039455-84-9

Batch: Purity:99.51
COTI-2 Chemical Structure
Chemical Structure
Check the COTI-2 product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

CAS Number 1039455-84-9
Molecular Formula

C19H22N6S

Molecular Weight (MW) 366.48
SMILES C1CC2=C(C(=NNC(=S)N3CCN(CC3)C4=CC=CC=N4)C1)N=CC=C2

Ordering Information

Biological Activity

COTI-2 is an orally available thiosemicarbazone derivative that acts as a targeted activator of mutant forms of p53 protein. By inducing conformational restoration and restoring wild-type p53 transcriptional activity, COTI-2 effectively reactivates downstream tumor suppressor cascades, suppresses AKT/mTOR signaling pathways, and selectively impairs cellular proliferation while triggering apoptosis in diverse p53-mutant cancer cell types. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 6.25 mg/mL (17.05 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.