CAS No. 477-30-5
Batch:
Purity:99.91
Chemical Structure
Check the
Colcemid
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | (7S)-1,2,3,10-tetramethoxy-7-(methylamino)-6,7-dihydro-5H-benzo[a]heptalen-9-one | |
|---|---|---|
| CAS Number | 477-30-5 | |
| Molecular Formula |
C21H25NO5 |
|
| Molecular Weight (MW) | 371.43 | |
| SMILES | CNC1CCC2=CC(=C(OC)C(=C2C3=CC=C(OC)C(=O)C=C13)OC)OC | |
| InChIKey | NNJPGOLRFBJNIW-HNNXBMFYSA-N |
Colcemid (Demecolcine) is a synthetic derivative of colchicine and a potent mitotic inhibitor that directly targets tubulin. By binding to tubulin, it depolymerizes microtubules and disrupts spindle apparatus formation during mitosis. This inhibition blocks cell cycle progression at the mitotic checkpoint, preventing proper chromosome segregation and triggering cell apoptosis in cancer research models.[1][2]
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Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
75 mg/mL
(201.92 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 75 mg/mL |
| Ethanol | 75 mg/mL |
| DMSO |
74 mg/mL
(199.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | 74 mg/mL |
| Ethanol | 74 mg/mL |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
Note: Technical data last updated: Sep 1, 2026.