CAS No. 2250025-93-3

Batch: Purity:97.62
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Clozapine N-oxide dihydrochloride Chemical Structure
Chemical Structure
Check the Clozapine N-oxide dihydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 3-chloro-6-(4-methyl-4-oxidopiperazin-4-ium-1-yl)-11H-benzo[b][1,4]benzodiazepine;dihydrochloride
CAS Number 2250025-93-3
Molecular Formula

C18H19ClN4O.2ClH
Molecular Weight (MW) 415.75
InChIKey MBRGKRXDVKTUPT-UHFFFAOYSA-N

Biological Activity

Clozapine N-oxide dihydrochloride is a primary metabolite of clozapine that acts as a synthetic agonist for human muscarinic designer receptors exclusively activated by designer drugs (DREADDs), with a pfor the M1 DREADD. Activation of G protein-coupled DREADDs triggers downstream Gq-mediated intracellular calcium mobilization, enabling precise chemogenetic control of neuronal excitability and signaling pathways in target cell populations. [1]

How to Prepare Stock Solution?

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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 83 mg/mL (199.63 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 20 mg/mL
Ethanol 20 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Armbruster BN, et al. Proc Natl Acad Sci U S A. 2007;104(12):5163-8.

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Note: Technical data last updated: Sep 1, 2026.