CAS No. 163252-36-6

Batch: Purity:99.96
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Clevudine Chemical Structure
Chemical Structure
Check the Clevudine product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 1-[(2S,3R,4S,5S)-3-fluoro-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
CAS Number 163252-36-6
Molecular Formula

C10H13FN2O5
Molecular Weight (MW) 260.22
SMILES CC1=CN(C(=O)NC1=O)C2C(C(C(O2)CO)O)F
InChIKey GBBJCSTXCAQSSJ-XQXXSGGOSA-N

Biological Activity

Clevudine (L-FMAU) is a synthetic pyrimidine nucleoside analog that acts as a potent inhibitor of hepatitis B virus (HBV) DNA polymerase. Upon intracellular phosphorylation to its active triphosphate form, it competitively inhibits viral reverse transcriptase and DNA polymerase, suppressing viral DNA synthesis and replication. This blockade impairs viral genome amplification without significant incorporation into host cellular DNA. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 52 mg/mL (199.83 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 52 mg/mL
Ethanol 4 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

Reference

[1] Chu CK, et al. Antimicrob Agents Chemother. 1995;39(4):979-81.

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Note: Technical data last updated: Sep 1, 2026.