CAS No. 470-37-1

Batch: Purity:99.93
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Cinobufagin Chemical Structure
Chemical Structure
Check the Cinobufagin product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name [(1R,2S,4R,5R,6R,7R,10S,11S,14S,16R)-14-hydroxy-7,11-dimethyl-6-(6-oxopyran-3-yl)-3-oxapentacyclo[8.8.0.02,4.02,7.011,16]octadecan-5-yl] acetate
CAS Number 470-37-1
Molecular Formula

C26H34O6
Molecular Weight (MW) 442.54
SMILES CC(=O)OC1C2OC23C4CCC5CC(O)CCC5(C)C4CCC3(C)C1C6=COC(=O)C=C6
InChIKey SCULJPGYOQQXTK-OLRINKBESA-N

Ordering Information

Biological Activity

Cinobufagin is a natural cardiotonic steroid and active component of Venenum Bufonis that exhibits potent antitumor activity. It inhibits PI3K, AKT, and Bcl-2 signaling while triggering upregulation of ATM and Chk2, leading to downregulation of CDC25C, CDK1, and cyclin B. These molecular alterations disrupt cell cycle progression, inducing G2/M phase arrest and activating cleaved caspase-9 and caspase-3 to promote apoptotic cell death. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (225.96 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.