CAS No. 3545-67-3

Batch: Purity:99.971
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Chloroquine dihydrochloride Chemical Structure
Chemical Structure
Check the Chloroquine dihydrochloride product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name 4-N-(7-chloroquinolin-4-yl)-1-N,1-N-diethylpentane-1,4-diamine;dihydrochloride
CAS Number 3545-67-3
Molecular Formula

C18H26ClN3.2ClH
Molecular Weight (MW) 392.79
InChIKey PCFGECQRSMVKCC-UHFFFAOYSA-N

Ordering Information

Biological Activity

Chloroquine dihydrochloride is an antimalarial and anti-inflammatory agent that functions as an inhibitor of autophagy and Toll-like receptors (TLRs). It prevents autophagic degradation by impairing lysosomal acidification and blocking fusion between autophagosomes and lysosomes. This disruption of autophagic flux leads to accumulation of autophagic vacuoles and suppresses cellular recycling under stress. Furthermore, inhibition of endosomal TLR pathways disrupts innate immune signaling and suppresses downstream pro-inflammatory cytokine production. [1]

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 71 mg/mL (180.75 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 71 mg/mL
Ethanol 71 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.